Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00562670" target="_blank" >RIV/61388963:_____/22:00562670 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/admi.202201453" target="_blank" >https://doi.org/10.1002/admi.202201453</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/admi.202201453" target="_blank" >10.1002/admi.202201453</a>
Alternative languages
Result language
angličtina
Original language name
Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray-Based Biosensing
Original language description
Diamond enables the construction of various (bio)sensors, including those with quantum-based detection. However, bare diamond interfaces are susceptible to unspecific adhesion of proteins and other macromolecules from biological media or complex samples. This impairs selectivity in biosensing, leads to low signal-to-noise ratio in fluorescence-based applications, and introduces the need for blocking steps in incubation protocols. Here, a stable, protein-repellent, and clickable reactive polymer coating is introduced, abolishing unspecific protein adhesion while concurrently enabling covalent immobilization of functional compounds as recognition elements. The polymer coating has two segments, an antifouling poly(N-(2-hydroxypropyl) methacrylamide) and an alkyne-terminated poly(propargyl methacrylamide) providing the click functionality. The antifouling properties and click-reactivity of the polymers are demonstrated by selective protein binding assays on micropatterns written by microchannel cantilever spotting (mu CS). The assays demonstrated the successful functionalization of both diamond and glass surfaces and the excellent antifouling properties of the polymer coating. The coating procedure is compatible with oxidized diamond surfaces thus well-suitable for diamond-based quantum technology. The results can directly impact applications of diamond materials in optically detected quantum sensing or fluorescence sensing in general. The polymer functionalization can also be used for any case where highly specific interaction with low fouling is desired.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20501 - Materials engineering
Result continuities
Project
<a href="/en/project/EF16_026%2F0008382" target="_blank" >EF16_026/0008382: Carbon allotropes with rationalized nanointerfaces and nanolinks for environmental and biomedical applications</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Advanced Materials Interfaces
ISSN
2196-7350
e-ISSN
2196-7350
Volume of the periodical
9
Issue of the periodical within the volume
33
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
2201453
UT code for WoS article
000865567900001
EID of the result in the Scopus database
2-s2.0-85139453225