Clickable antifouling polymer brushes for polymer pen lithography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F17%3A00473863" target="_blank" >RIV/61389013:_____/17:00473863 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/acsami.7b01184" target="_blank" >http://dx.doi.org/10.1021/acsami.7b01184</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.7b01184" target="_blank" >10.1021/acsami.7b01184</a>

Result language

angličtina

Original language name

Clickable antifouling polymer brushes for polymer pen lithography

Original language description

Protein-repellent reactive surfaces that promote localized specific binding are highly desirable for applications in the biomedical field. Nonspecific adhesion will compromise the function of bioactive surfaces, leading to ambiguous results of binding assays and negating the binding specificity of patterned cell-adhesive motives. Localized specific binding is often achieved by attaching a linker to the surface, and the other side of the linker is used to bind specifically to a desired functional agent, as e.g. proteins, antibodies, and fluorophores, depending on the function required by the application. We present a protein-repellent polymer brush enabling highly specific covalent surface immobilization of biorecognition elements by strain-promoted alkyne–azide cycloaddition click chemistry for selective protein adhesion. The protein-repellent polymer brush is functionalized by highly localized molecular binding sites in the low micrometer range using polymer pen lithography (PPL). Because of the massive parallelization of writing pens, the tunable PPL printed patterns can span over square centimeter areas. The selective binding of the protein streptavidin to these surface sites is demonstrated while the remaining polymer brush surface is resisting nonspecific adsorption without any prior blocking by bovine serum albumin (BSA). In contrast to the widely used BSA blocking, the reactive polymer brushes are able to significantly reduce nonspecific protein adsorption, which is the cause of biofouling. This was achieved for solutions of single proteins as well as complex biological fluids. The remarkable fouling resistance of the polymer brushes has the potential to improve the multiplexing capabilities of protein probes and therefore impact biomedical research and applications.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

<a href="/en/project/GJ15-09368Y" target="_blank" >GJ15-09368Y: Unraveling the physicochemical phenomena leading to antifouling bioactive surfaces</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS Applied Materials and Interfaces

ISSN

1944-8244

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

13

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

12109-12117

UT code for WoS article

000398764100089

EID of the result in the Scopus database

2-s2.0-85017142845