Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00563508" target="_blank" >RIV/61388963:_____/22:00563508 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10447060
Result on the web
<a href="https://doi.org/10.3390/mps5050070" target="_blank" >https://doi.org/10.3390/mps5050070</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/mps5050070" target="_blank" >10.3390/mps5050070</a>
Alternative languages
Result language
angličtina
Original language name
Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune Cells
Original language description
The liver is a complex organ that governs many types of metabolisms, including energy metabolism and other cellular processes. The liver also plays a crucial role in important functions in immunity, and the activity of liver tissue-associated immunity affects the outcome of many liver pathologies. A thorough characterization of the liver immune microenvironment may contribute to a better understanding of immune signaling, the mechanisms of specific immune responses, and even to improved predictions about therapy outcomes. In this paper, we present an optimized, simple, and rapid protocol to characterize the liver-associated immune cell milieu. We believe that the most suitable technique for obtaining a complex immune cell suspension and for removing contaminating blood cells is to perform mouse liver perfusion, using only phosphate buffer saline. Combining an enzymatic digestion and a mechanical dissociation of liver tissue, followed by cell purification, improves downstream applications. This combination is an essential prerequisite for immune cell determination and characterization. We then demonstrate a flow cytometry-based multiparametric immunophenotyping along with a gating strategy to detect and quantify liver endothelial cells, T cells (helper and cytotoxic), B cells, NK cells, NKT cells, neutrophils, monocytes (subsets included), dendritic cells (subsets included), macrophages and Kupffer cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemical biology for drugging undruggable targets</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods and Protocols
ISSN
2409-9279
e-ISSN
2409-9279
Volume of the periodical
5
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
70
UT code for WoS article
000873086400001
EID of the result in the Scopus database
2-s2.0-85140616880