The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00571293" target="_blank" >RIV/61388963:_____/23:00571293 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/23:00079226 RIV/00216208:11310/23:10467035
Result on the web
<a href="https://doi.org/10.1038/s42004-023-00862-0" target="_blank" >https://doi.org/10.1038/s42004-023-00862-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42004-023-00862-0" target="_blank" >10.1038/s42004-023-00862-0</a>
Alternative languages
Result language
angličtina
Original language name
The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70
Original language description
Nucleic acids aptamers often fail to efficiently target some proteins because of the hydrophilic character of the natural nucleotides. Here we present hydrophobic 7-phenylbutyl-7-deaadenine-modified DNA aptamers against the Heat Shock Protein 70 that were selected via PEX and magnetic bead-based SELEX. After 9 rounds of selection, the pool was sequenced and a number of candidates were identified. Following initial screening, two modified aptamers were chemically synthesised in-house and their binding affinity analysed by two methods, bio-layer interferometry and fluorescent-plate-based binding assay. The binding affinities of the modified aptamers were compared with that of their natural counterparts. The resulting modified aptamers bound with higher affinity (low nanomolar range) to the Hsp70 than their natural sequence (>5 µM) and hence have potential for applications and further development towards Hsp70 diagnostics or even therapeutics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Communications Chemistry
ISSN
2399-3669
e-ISSN
2399-3669
Volume of the periodical
6
Issue of the periodical within the volume
April
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
65
UT code for WoS article
000964139800002
EID of the result in the Scopus database
2-s2.0-85152694501