Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00574341" target="_blank" >RIV/61388963:_____/23:00574341 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10474263
Result on the web
<a href="https://doi.org/10.1021/acs.analchem.3c02023" target="_blank" >https://doi.org/10.1021/acs.analchem.3c02023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.3c02023" target="_blank" >10.1021/acs.analchem.3c02023</a>
Alternative languages
Result language
angličtina
Original language name
Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition
Original language description
The previously reported approach of orthogonal multipotential redox coding of all four DNA bases allowed only analysis of the relative nucleotide composition of short DNA stretches. Here, we present two methods for normalization of the electrochemical readout to facilitate the determination of the total nucleotide composition. The first method is based on the presence or absence of an internal standard of 7-deaza-2′-deoxyguanosine in a DNA primer. The exact composition of the DNA was elucidated upon two parallel analyses and the subtraction of the electrochemical signal intensities. The second approach took advantage of a 5′-viologen modified primer, with this fifth orthogonal redox label acting as a reference for signal normalization, thus allowing accurate electrochemical sequence analysis in a single read. Both approaches were tested using various sequences, and the voltammetric signals obtained were normalized using either the internal standard or the reference label and demonstrated to be in perfect agreement with the actual nucleotide composition, highlighting the potential for targeted DNA sequence analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
1520-6882
Volume of the periodical
95
Issue of the periodical within the volume
34
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
12586-12589
UT code for WoS article
001050912800001
EID of the result in the Scopus database
2-s2.0-85169028174