Phenothiazine-linked nucleosides and nucleotides for redox labelling of DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00479760" target="_blank" >RIV/61388963:_____/17:00479760 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/17:00479760 RIV/00216224:14740/17:00095595 RIV/00216208:11310/17:10367339
Result on the web
<a href="http://pubs.rsc.org/en/content/articlehtml/2017/ob/c7ob01439b" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2017/ob/c7ob01439b</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c7ob01439b" target="_blank" >10.1039/c7ob01439b</a>

Result language
angličtina
Original language name
Phenothiazine-linked nucleosides and nucleotides for redox labelling of DNA
Original language description
Nucleosides and 2'-deoxyribonucleoside triphosphates (dNTPs) bearing phenothiazine (PT) attached to a nucleobase (cytosine or 7-deazaadenine) either directly or through an acetylene linker were prepared through Suzuki or Sonogashira cross-coupling and triphosphorylation, and were studied as building blocks for polymerase construction of modified DNA. The directly PT-substituted dNTPs were better substrates for polymerases than the alkyne-linked dNTPs but all of them were used in enzymatic synthesis of DNA using primer extension, nicking enzyme amplification, PCR or 3'-tail labelling by terminal deoxynucleotidyl transferase. The phenothiazine served as an oxidizable redox label (giving two analytically useful signals of oxidation on electrode) for nucleosides and DNA and was also used in orthogonal combination with previously developed benzofurazane or nitrophenyl labels for redox coding of DNA bases. Therefore, the title PT-linked dNTPs are useful additions to the portfolio of nucleotides for enzymatic synthesis of redox-labelled DNA for electrochemical analysis.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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