Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00575321" target="_blank" >RIV/61388963:_____/23:00575321 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/23:00572141 RIV/61388971:_____/23:00575321 RIV/00216208:11110/23:10465309 RIV/49777513:23520/23:43971197
Result on the web
<a href="https://www.mdpi.com/2218-1989/13/4/552" target="_blank" >https://www.mdpi.com/2218-1989/13/4/552</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/metabo13040552" target="_blank" >10.3390/metabo13040552</a>

Result language
angličtina
Original language name
Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis
Original language description
Zucker fatty (fa/fa) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young fa/fa rats up to 20 weeks of age, which can be considered early maturity in male fa/fa rats, the aim of our work was to extend the metabolomic characterization to significantly older animals. Therefore, the urinary profiles of obese fa/fa rats and their lean controls were monitored using untargeted NMR metabolomics between 12 and 40 weeks of age. At the end of the experiment, the rats were also characterized by NMR and LC-MS serum analysis, which was supplemented by a targeted LC-MS analysis of serum bile acids and neurotransmitters. The urine analysis showed that most of the characteristic differences detected in young obese fa/fa rats persisted throughout the experiment, primarily through a decrease in microbial co-metabolite levels, the upregulation of the citrate cycle, and changes in nicotinamide metabolism compared with the age-related controls. The serum of 40-week-old obese rats showed a reduction in several bile acid conjugates and an increase in serotonin. Our study demonstrated that the fa/fa model of genetic obesity is stable up to 40 weeks of age and is therefore suitable for long-term experiments.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)

Project
<a href="/en/project/GA20-00546S" target="_blank" >GA20-00546S: Obesity, diabetes and neurodegeneration crosstalk: New therapeutic potential of prolactin-releasing peptide analogs</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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