On-tissue dataset-dependent MALDI-TIMS-MS2 bioimaging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00578317" target="_blank" >RIV/61388963:_____/23:00578317 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s41467-023-43298-9" target="_blank" >https://doi.org/10.1038/s41467-023-43298-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-023-43298-9" target="_blank" >10.1038/s41467-023-43298-9</a>
Alternative languages
Result language
angličtina
Original language name
On-tissue dataset-dependent MALDI-TIMS-MS2 bioimaging
Original language description
Trapped ion mobility spectrometry (TIMS) adds an additional separation dimension to mass spectrometry (MS) imaging, however, the lack of fragmentation spectra (MS2) impedes confident compound annotation in spatial metabolomics. Here, we describe spatial ion mobility-scheduled exhaustive fragmentation (SIMSEF), a dataset-dependent acquisition strategy that augments TIMS-MS imaging datasets with MS2 spectra. The fragmentation experiments are systematically distributed across the sample and scheduled for multiple collision energies per precursor ion. Extendable data processing and evaluation workflows are implemented into the open source software MZmine. The workflow and annotation capabilities are demonstrated on rat brain tissue thin sections, measured by matrix-assisted laser desorption/ionisation (MALDI)-TIMS-MS, where SIMSEF enables on-tissue compound annotation through spectral library matching and rule-based lipid annotation within MZmine and maps the (un)known chemical space by molecular networking. The SIMSEF algorithm and data analysis pipelines are open source and modular to provide a community resource.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GM21-11563M" target="_blank" >GM21-11563M: Mapping the chemodiversity of Piperaceae plants using next-generation MZmine platform</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
2041-1723
Volume of the periodical
14
Issue of the periodical within the volume
November
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
7495
UT code for WoS article
001106858800048
EID of the result in the Scopus database
2-s2.0-85176926137