High-throughput Selection of Human de novo-emerged sORFs with High Folding Potential
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585603" target="_blank" >RIV/61388963:_____/24:00585603 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10483116
Result on the web
<a href="https://doi.org/10.1093/gbe/evae069" target="_blank" >https://doi.org/10.1093/gbe/evae069</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/gbe/evae069" target="_blank" >10.1093/gbe/evae069</a>
Alternative languages
Result language
angličtina
Original language name
High-throughput Selection of Human de novo-emerged sORFs with High Folding Potential
Original language description
De novo genes emerge from previously noncoding stretches of the genome. Their encoded de novo proteins are generally expected to be similar to random sequences and, accordingly, with no stable tertiary fold and high predicted disorder. However, structural properties of de novo proteins and whether they differ during the stages of emergence and fixation have not been studied in depth and rely heavily on predictions. Here we generated a library of short human putative de novo proteins of varying lengths and ages and sorted the candidates according to their structural compactness and disorder propensity. Using Forster resonance energy transfer combined with Fluorescence-activated cell sorting, we were able to screen the library for most compact protein structures, as well as most elongated and flexible structures. We find that compact de novo proteins are on average slightly shorter and contain lower predicted disorder than less compact ones. The predicted structures for most and least compact de novo proteins correspond to expectations in that they contain more secondary structure content or higher disorder content, respectively. Our experiments indicate that older de novo proteins have higher compactness and structural propensity compared with young ones. We discuss possible evolutionary scenarios and their implications underlying the age-dependencies of compactness and structural content of putative de novo proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GB14-36098G" target="_blank" >GB14-36098G: Center for tropical biology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genome Biology and Evolution
ISSN
1759-6653
e-ISSN
1759-6653
Volume of the periodical
16
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
evae069
UT code for WoS article
001204479300001
EID of the result in the Scopus database
2-s2.0-85191082529