Simple, fast, cost-efficient, reliable, and highly automated DNA content analysis of cells in adherent cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585791" target="_blank" >RIV/61388963:_____/24:00585791 - isvavai.cz</a>
Alternative codes found
RIV/75010330:_____/24:00014696 RIV/60162694:G44__/25:00563332 RIV/00216208:11110/24:10482396 RIV/00064211:_____/24:W0000011
Result on the web
<a href="https://doi.org/10.1002/cyto.a.24840" target="_blank" >https://doi.org/10.1002/cyto.a.24840</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.24840" target="_blank" >10.1002/cyto.a.24840</a>
Alternative languages
Result language
angličtina
Original language name
Simple, fast, cost-efficient, reliable, and highly automated DNA content analysis of cells in adherent cultures
Original language description
The most commonly used flow cytometric (FCM) analysis of cellular DNA content relies on ethanol fixation followed by RNA digestion and propidium iodide (PI) intercalation into double-stranded DNA. This is a laborious and time-consuming procedure that is subject to systematic errors due to centrifugation and washing steps associated with sample preparation. It can adversely affect the reliability of the results. Here, we present a modified concept of DNA quantification in adherent cell lines by FCM that involves neither ethanol fixation nor any washing and cell transferring steps. Our high throughput assay of adherent cell lines reduces sample-processing time, requires minimal workload, provides a possibility for automation, and, if needed, also allows a significant reduction in the size of individual samples. Working with a well-proven commercial tool-The BD Cycletest Plus DNA Reagent Kit-primarily designed for cell cycle analysis and aneuploidy determination in experimental and clinical samples, we suggest a novel, very efficient, and robust approach for DNA research in adherent cell cultures.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry. Part A
ISSN
1552-4922
e-ISSN
1552-4930
Volume of the periodical
105
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
474-479
UT code for WoS article
001217258600001
EID of the result in the Scopus database
2-s2.0-85192204537