Iridium-based polymeric multifunctional imaging tools for biochemistry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585892" target="_blank" >RIV/61388963:_____/24:00585892 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/24:00585892 RIV/00216208:11110/24:10475817
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cplu.202300647" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cplu.202300647</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cplu.202300647" target="_blank" >10.1002/cplu.202300647</a>
Alternative languages
Result language
angličtina
Original language name
Iridium-based polymeric multifunctional imaging tools for biochemistry
Original language description
Herein, we report the development of a macromolecular multifunctional imaging tool for biological investigations, which is comprised of an N-(2-hydroxypropyl)methacrylamide backbone, iridium-based luminescent probe, glutamate carboxypeptidase II (GCPII) targeting ligand, and biotin affinity tag. The iridium luminophore is a tris-cyclometalated complex based on [Ir(ppy)3] with one of its 2-phenylpyridine ligands functionalized to allow conjugation. Synthesized macromolecular probes differed in the structure of the polymer and content of the iridium complex. The applicability of the developed imaging tools has been tested in flow cytometry (FACS) based assay, laser confocal microscopy, and fluorescence lifetime imaging microscopy (FLIM). The FACS analysis has shown that the targeted iBodies containing the iridium luminophore exhibit selective labelling of GCPII expressing cells. This observation was also confirmed in the imaging experiments with laser confocal microscopy. The FLIM experiment has shown that the iBodies with the iridium label exhibit a lifetime greater than 100 ns, which distinguishes them from typically used systems labelled with organic fluorophores exhibiting short fluorescence lifetimes. The results of this investigation indicate that the system exhibits interesting properties, which supports the development of additional biological tools utilizing the key components (iridium complexes, iBody concept), primarily focusing on the longer lifetime of the iridium emitter.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30107 - Medicinal chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemPlusChem
ISSN
2192-6506
e-ISSN
2192-6506
Volume of the periodical
89
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
e202300647
UT code for WoS article
001147392100001
EID of the result in the Scopus database
2-s2.0-85182823582