HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585901" target="_blank" >RIV/61388963:_____/24:00585901 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/24:00585901 RIV/60076658:12310/24:43908872 RIV/00216208:11110/24:10482650 RIV/00216208:11310/24:10482650
Result on the web
<a href="https://doi.org/10.1021/acschembio.4c00151" target="_blank" >https://doi.org/10.1021/acschembio.4c00151</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acschembio.4c00151" target="_blank" >10.1021/acschembio.4c00151</a>
Alternative languages
Result language
angličtina
Original language name
HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA
Original language description
Nicotinamide adenine dinucleotide (NAD) is a critical component of the cellular metabolism and also serves as an alternative 5′ cap on various RNAs. However, the function of the NAD RNA cap is still under investigation. We studied NAD capping of RNAs in HIV-1-infected cells because HIV-1 is responsible for the depletion of the NAD/NADH cellular pool and causing intracellular pellagra. By applying the NAD captureSeq protocol to HIV-1-infected and uninfected cells, we revealed that four snRNAs (e.g., U1) and four snoRNAs lost their NAD cap when infected with HIV-1. Here, we provide evidence that the presence of the NAD cap decreases the stability of the U1/HIV-1 pre-mRNA duplex. Additionally, we demonstrate that reducing the quantity of NAD-capped RNA by overexpressing the NAD RNA decapping enzyme DXO results in an increase in HIV-1 infectivity. This suggests that NAD capping is unfavorable for HIV-1 and plays a role in its infectivity.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Chemical Biology
ISSN
1554-8929
e-ISSN
1554-8937
Volume of the periodical
19
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1243-1249
UT code for WoS article
001226092100001
EID of the result in the Scopus database
2-s2.0-85193526644