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CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5 ' Capping with NAD(+)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F18%3A10389190" target="_blank" >RIV/00216208:11320/18:10389190 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/18:00490124

  • Result on the web

    <a href="https://doi.org/10.1016/j.molcel.2018.03.014" target="_blank" >https://doi.org/10.1016/j.molcel.2018.03.014</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.molcel.2018.03.014" target="_blank" >10.1016/j.molcel.2018.03.014</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5 ' Capping with NAD(+)

  • Original language description

    Nucleoside-containing metabolites such asNAD(+) can be incorporated as 50 caps on RNA by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase (RNAP). Here, we report CapZyme-seq, a high-throughput-sequencing method that employs NCIN-decapping enzymes NudC and Rai1 to detect and quantify NCIN-capped RNA. By combining CapZyme-seq with multiplexed transcriptomics, we determine efficiencies of NAD(+) capping by Escherichia coli RNAP for similar to 16,000 promoter sequences. The results define preferred transcription start site (TSS) positions for NAD(+) capping and define a consensus promoter sequence for NAD(+) capping: HRRASWW (TSS underlined). By applying CapZyme-seq to E. coli total cellular RNA, we establish that sequence determinants for NCIN capping in vivo match the NAD(+)-capping consensus defined in vitro, and we identify and quantify NCIN-capped small RNAs (sRNAs). Our findings define the promoter-sequence determinants for NCIN capping with NAD(+) and provide a general method for analysis of NCIN capping in vitro and in vivo.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/GA15-05228S" target="_blank" >GA15-05228S: Deciphering the Cellular Role of HelD, a Helicase-like Protein Associated with Bacterial RNA Polymerase.</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular Cell

  • ISSN

    1097-2765

  • e-ISSN

  • Volume of the periodical

    70

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    553-564

  • UT code for WoS article

    000432517300015

  • EID of the result in the Scopus database

    2-s2.0-85045536565