CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5 ' Capping with NAD(+)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F18%3A10389190" target="_blank" >RIV/00216208:11320/18:10389190 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00490124
Result on the web
<a href="https://doi.org/10.1016/j.molcel.2018.03.014" target="_blank" >https://doi.org/10.1016/j.molcel.2018.03.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molcel.2018.03.014" target="_blank" >10.1016/j.molcel.2018.03.014</a>
Alternative languages
Result language
angličtina
Original language name
CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5 ' Capping with NAD(+)
Original language description
Nucleoside-containing metabolites such asNAD(+) can be incorporated as 50 caps on RNA by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase (RNAP). Here, we report CapZyme-seq, a high-throughput-sequencing method that employs NCIN-decapping enzymes NudC and Rai1 to detect and quantify NCIN-capped RNA. By combining CapZyme-seq with multiplexed transcriptomics, we determine efficiencies of NAD(+) capping by Escherichia coli RNAP for similar to 16,000 promoter sequences. The results define preferred transcription start site (TSS) positions for NAD(+) capping and define a consensus promoter sequence for NAD(+) capping: HRRASWW (TSS underlined). By applying CapZyme-seq to E. coli total cellular RNA, we establish that sequence determinants for NCIN capping in vivo match the NAD(+)-capping consensus defined in vitro, and we identify and quantify NCIN-capped small RNAs (sRNAs). Our findings define the promoter-sequence determinants for NCIN capping with NAD(+) and provide a general method for analysis of NCIN capping in vitro and in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA15-05228S" target="_blank" >GA15-05228S: Deciphering the Cellular Role of HelD, a Helicase-like Protein Associated with Bacterial RNA Polymerase.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Cell
ISSN
1097-2765
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
553-564
UT code for WoS article
000432517300015
EID of the result in the Scopus database
2-s2.0-85045536565