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Dinucleoside polyphosphates act as 5′-RNA caps in bacteria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00522578" target="_blank" >RIV/61388963:_____/20:00522578 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/20:00522578

  • Result on the web

    <a href="https://www.nature.com/articles/s41467-020-14896-8" target="_blank" >https://www.nature.com/articles/s41467-020-14896-8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41467-020-14896-8" target="_blank" >10.1038/s41467-020-14896-8</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dinucleoside polyphosphates act as 5′-RNA caps in bacteria

  • Original language description

    It has been more than 50 years since the discovery of dinucleoside polyphosphates (NpnNs) and yet their roles and mechanisms of action remain unclear. Here, we show that both methylated and non-methylated NpnNs serve as RNA caps in Escherichia coli. NpnNs are excellent substrates for T7 and E. coli RNA polymerases (RNAPs) and efficiently initiate transcription. We demonstrate, that the E. coli enzymes RNA 5′-pyrophosphohydrolase (RppH) and bis(5′-nucleosyl)-tetraphosphatase (ApaH) are able to remove the NpnN-caps from RNA. ApaH is able to cleave all NpnN-caps, while RppH is unable to cleave the methylated forms suggesting that the methylation adds an additional layer to RNA stability regulation. Our work introduces a different perspective on the chemical structure of RNA in prokaryotes and on the role of RNA caps. We bring evidence that small molecules, such as NpnNs are incorporated into RNA and may thus influence the cellular metabolism and RNA turnover.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nature Communications

  • ISSN

    2041-1723

  • e-ISSN

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    Feb 26

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    1052

  • UT code for WoS article

    000564290500006

  • EID of the result in the Scopus database

    2-s2.0-85080053995