Dinucleoside polyphosphates act as 5′-RNA caps in bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00522578" target="_blank" >RIV/61388963:_____/20:00522578 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/20:00522578
Result on the web
<a href="https://www.nature.com/articles/s41467-020-14896-8" target="_blank" >https://www.nature.com/articles/s41467-020-14896-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-020-14896-8" target="_blank" >10.1038/s41467-020-14896-8</a>
Alternative languages
Result language
angličtina
Original language name
Dinucleoside polyphosphates act as 5′-RNA caps in bacteria
Original language description
It has been more than 50 years since the discovery of dinucleoside polyphosphates (NpnNs) and yet their roles and mechanisms of action remain unclear. Here, we show that both methylated and non-methylated NpnNs serve as RNA caps in Escherichia coli. NpnNs are excellent substrates for T7 and E. coli RNA polymerases (RNAPs) and efficiently initiate transcription. We demonstrate, that the E. coli enzymes RNA 5′-pyrophosphohydrolase (RppH) and bis(5′-nucleosyl)-tetraphosphatase (ApaH) are able to remove the NpnN-caps from RNA. ApaH is able to cleave all NpnN-caps, while RppH is unable to cleave the methylated forms suggesting that the methylation adds an additional layer to RNA stability regulation. Our work introduces a different perspective on the chemical structure of RNA in prokaryotes and on the role of RNA caps. We bring evidence that small molecules, such as NpnNs are incorporated into RNA and may thus influence the cellular metabolism and RNA turnover.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
Feb 26
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
1052
UT code for WoS article
000564290500006
EID of the result in the Scopus database
2-s2.0-85080053995