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Arabidopsis thaliana NudiXes have RNA-decapping activity

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00569480" target="_blank" >RIV/61388963:_____/23:00569480 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/23:10469613

  • Result on the web

    <a href="https://doi.org/10.1039/D2CB00213B" target="_blank" >https://doi.org/10.1039/D2CB00213B</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1039/d2cb00213b" target="_blank" >10.1039/d2cb00213b</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Arabidopsis thaliana NudiXes have RNA-decapping activity

  • Original language description

    Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (NpnN), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped with Np(n)Ns (Ap(2-5)A, Gp(3-4)G, Ap(3-5)G, m(7)Gp(3)G, m(7)Gp(3)A), CoA, ADP-ribose, or NAD(H). While AtNUDT19 preferred NADH-RNA over other types of capped RNA, AtNUDT6 and AtNUDT7 preferentially cleaved Ap(4)A-RNA. The most powerful decapping enzyme was AtNUDT27, which cleaved almost all types of capped RNA at a tenfold lower concentration than the other enzymes. We also compared cleavage efficiency of each enzyme on free small molecules with RNA capped with corresponding molecules. We found that AtNUDT6 prefers free Ap(4)A, while AtNUDT7 preferentially cleaved Ap(4)A-RNA. These findings show that NudiX enzymes may act as RNA-decapping enzymes in A. thaliana and that other noncanonical RNA caps such as Ap(4)A and NADH should be searched for in plant RNA.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LL1603" target="_blank" >LL1603: Viral RNA modifications – Essential steps in Chemical Evolution of Protein Cofactors</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    RSC Chemical Biology

  • ISSN

    2633-0679

  • e-ISSN

    2633-0679

  • Volume of the periodical

    4

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    6

  • Pages from-to

    223-228

  • UT code for WoS article

    000915348600001

  • EID of the result in the Scopus database

    2-s2.0-85150293104