Arabidopsis thaliana NudiXes have RNA-decapping activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00569480" target="_blank" >RIV/61388963:_____/23:00569480 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10469613
Result on the web
<a href="https://doi.org/10.1039/D2CB00213B" target="_blank" >https://doi.org/10.1039/D2CB00213B</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d2cb00213b" target="_blank" >10.1039/d2cb00213b</a>
Alternative languages
Result language
angličtina
Original language name
Arabidopsis thaliana NudiXes have RNA-decapping activity
Original language description
Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (NpnN), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped with Np(n)Ns (Ap(2-5)A, Gp(3-4)G, Ap(3-5)G, m(7)Gp(3)G, m(7)Gp(3)A), CoA, ADP-ribose, or NAD(H). While AtNUDT19 preferred NADH-RNA over other types of capped RNA, AtNUDT6 and AtNUDT7 preferentially cleaved Ap(4)A-RNA. The most powerful decapping enzyme was AtNUDT27, which cleaved almost all types of capped RNA at a tenfold lower concentration than the other enzymes. We also compared cleavage efficiency of each enzyme on free small molecules with RNA capped with corresponding molecules. We found that AtNUDT6 prefers free Ap(4)A, while AtNUDT7 preferentially cleaved Ap(4)A-RNA. These findings show that NudiX enzymes may act as RNA-decapping enzymes in A. thaliana and that other noncanonical RNA caps such as Ap(4)A and NADH should be searched for in plant RNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LL1603" target="_blank" >LL1603: Viral RNA modifications – Essential steps in Chemical Evolution of Protein Cofactors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RSC Chemical Biology
ISSN
2633-0679
e-ISSN
2633-0679
Volume of the periodical
4
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
223-228
UT code for WoS article
000915348600001
EID of the result in the Scopus database
2-s2.0-85150293104