Apollon: a deoxyribozyme that generates a yellow product
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00586924" target="_blank" >RIV/61388963:_____/24:00586924 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10485175
Result on the web
<a href="https://doi.org/10.1093/nar/gkae490" target="_blank" >https://doi.org/10.1093/nar/gkae490</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkae490" target="_blank" >10.1093/nar/gkae490</a>
Alternative languages
Result language
angličtina
Original language name
Apollon: a deoxyribozyme that generates a yellow product
Original language description
Colorimetric assays in which the color of a solution changes in the presence of an input provide a simple and inexpensive way to monitor experimental readouts. In this study we used in vitro selection to identify a self-phosphorylating kinase deoxyribozyme that produces a colorimetric signal by converting the colorless substrate pNPP into the yellow product pNP. The minimized catalytic core, sequence requirements, secondary structure, and buffer requirements of this deoxyribozyme, which we named Apollon, were characterized using a variety of techniques including reselection experiments, high-throughput sequencing, comparative analysis, biochemical activity assays, and NMR. A bimolecular version of Apollon catalyzed multiple turnover phosphorylation and amplified the colorimetric signal. Engineered versions of Apollon could detect oligonucleotides with specific sequences as well as several different types of nucleases in homogenous assays that can be performed in a single tube without the need for washes or purifications. We anticipate that Apollon will be particularly useful to reduce costs in high-throughput screens and for applications in which specialized equipment is not available.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
1362-4962
Volume of the periodical
52
Issue of the periodical within the volume
15
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
9062-9075
UT code for WoS article
001244910900001
EID of the result in the Scopus database
2-s2.0-85202694770