Optimizing the Chemiluminescence of a Light-Producing Deoxyribozyme
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556678" target="_blank" >RIV/61388963:_____/22:00556678 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10446953
Result on the web
<a href="https://doi.org/10.1002/cbic.202200026" target="_blank" >https://doi.org/10.1002/cbic.202200026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.202200026" target="_blank" >10.1002/cbic.202200026</a>
Alternative languages
Result language
angličtina
Original language name
Optimizing the Chemiluminescence of a Light-Producing Deoxyribozyme
Original language description
Supernova is a chemiluminescent deoxyribozyme recently discovered in our group. It transfers the phosphate group from the 1,2-dioxetane substrate CDP-Star to its 5 ' hydroxyl group, which triggers a decomposition reaction and the production of light. Here we investigated the effects of reaction conditions on the ability of Supernova to generate a chemiluminescent signal (using a plate reader assay) and to phosphorylate itself (using a ligation assay). Our experiments indicate that multiple zinc ions are required for catalytic function, suggesting links between Supernova and protein enzymes that catalyze similar reactions. They also show how factors such as pH, potassium concentration, CDP-Star concentration, and DNA concentration affect the reaction. By combining information from different experiments, the rate enhancement of light production was increased by more than 1000-fold. These results should be useful for applications in which Supernova is used as a sensor.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA19-20989S" target="_blank" >GA19-20989S: Using DNA to generate light</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
1439-7633
Volume of the periodical
23
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
e202200026
UT code for WoS article
000775093700001
EID of the result in the Scopus database
2-s2.0-85127512464