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Supernova: A Deoxyribozyme that Catalyzes a Chemiluminescent Reaction

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00551188" target="_blank" >RIV/61388963:_____/22:00551188 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/22:10457215

  • Result on the web

    <a href="https://doi.org/10.1002/anie.202109347" target="_blank" >https://doi.org/10.1002/anie.202109347</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/anie.202109347" target="_blank" >10.1002/anie.202109347</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Supernova: A Deoxyribozyme that Catalyzes a Chemiluminescent Reaction

  • Original language description

    Functional DNA molecules are useful components in nanotechnology and synthetic biology. To expand the toolkit of functional DNA parts, in this study we used artificial evolution to identify a glowing deoxyribozyme called Supernova. This deoxyribozyme transfers a phosphate from a 1,2-dioxetane substrate to its 5′ hydroxyl group, which triggers a chemiluminescent reaction and a flash of blue light. An engineered version of Supernova is only catalytically active in the presence of an oligonucleotide complementary to its 3′ end, demonstrating that light production can be coupled to ligand binding. We anticipate that Supernova will be useful in a wide variety of applications, including as a signaling component in allosterically regulated sensors and in logic gates of molecular computers.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Angewandte Chemie - International Edition

  • ISSN

    1433-7851

  • e-ISSN

    1521-3773

  • Volume of the periodical

    61

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    6

  • Pages from-to

    e202109347

  • UT code for WoS article

    000722279100001

  • EID of the result in the Scopus database

    2-s2.0-85120631774