Contact lenses as a potential vehicle of Candida transmission

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00587002" target="_blank" >RIV/61388963:_____/24:00587002 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10493829 RIV/00216275:25310/24:39921525

Result on the web

<a href="https://doi.org/10.1016/j.clae.2024.102249" target="_blank" >https://doi.org/10.1016/j.clae.2024.102249</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.clae.2024.102249" target="_blank" >10.1016/j.clae.2024.102249</a>

Result language

angličtina

Original language name

Contact lenses as a potential vehicle of Candida transmission

Original language description

Purpose: Contact lenses can be contaminated with various microorganisms, including pathogenic yeasts of the genus Candida, which are known for their ability to adhere to abiotic surfaces, including plastic materials used for various medical purposes. Microbial contamination of the lenses can lead to infection of the wearer's eyes. The purpose of this study was to simulate the contamination of contact lenses with C. albicans and C. parapsilosis, analyze the interaction of the microorganisms with the lens material, and optimize the protocol for PCR-based analysis of the microbial agents responsible for lens contamination. Methods: Hilafilcon lenses were exposed to C. albicans and C. parapsilosis cultures, washed, and examined for their ability to further spread the contamination. Scanning electron microscopy was used to analyze the attachment of yeast cells to the lenses. Infrared spectroscopy was used to examine the potential changes in the lens material due to Candida contamination. The protocol for DNA isolation from contaminated lenses was established to enable PCR analysis of microbes attached to the lenses. Results: Hilafilcon lenses contaminated with Candida were able to spread the contamination even after washing with saline or with a commercial cleaning solution. In the present experimental settings, the yeasts did not grow into the lenses but began to form biofilms on the surface. However, the ability of the lenses to retain water was altered. The PCR-based protocol could be used to help identify the type of contamination of contact lenses. Conclusion: Once contaminated with Candida albicans or Candida parapsilosis, Hilafilcon contact lenses are difficult to clean. Yeasts began to form biofilms on lens surfaces.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Contact Lens and Anterior Eye

ISSN

1367-0484

e-ISSN

1476-5411

Volume of the periodical

47

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

102249

UT code for WoS article

001315202000001

EID of the result in the Scopus database

2-s2.0-85195039894