NMR screen reveals the diverse structural landscape of a G-quadruplex library

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00598148" target="_blank" >RIV/61388963:_____/24:00598148 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10490216 RIV/00216208:11320/24:10490216

Result on the web

<a href="https://doi.org/10.1002/chem.202401437" target="_blank" >https://doi.org/10.1002/chem.202401437</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.202401437" target="_blank" >10.1002/chem.202401437</a>

Result language

angličtina

Original language name

NMR screen reveals the diverse structural landscape of a G-quadruplex library

Original language description

G-quadruplexes are noncanonical nucleic acid structures formed by stacked guanine tetrads.Despite their functional and structural diversity, a single consensus model is typically used to describe sequences with the potential to form G-quadruplex structures. We are interested in developing more specific sequence models for G-quadruplexes. In previous work, we functionally characterized each sequence in a 496-member library of variants of a monomeric reference G-quadruplex for the ability to bind GTP, promote a model peroxidase reaction, generate intrinsic fluorescence, and to form multimers. Here we used NMR to obtain a broad overview of the structural features of this library. After determining the1H NMR spectrum of each of these 496 sequences, spectra were sorted into multiple classes, most of which could be rationalized based on mutational patterns in the primary sequence. A more detailed screen using representative sequences provided additional information about spectral classes, and confirmed that the classes determined based on analysis of1H NMR spectra are correlated with functional categories identified in previous studies. These results provide new insights into the surprising structural diversity of this library. They also show how NMR can be used to identify classes of sequences with distinct mutational signatures and functions.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

1521-3765

Volume of the periodical

30

Issue of the periodical within the volume

67

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

e202401437

UT code for WoS article

001354202900001

EID of the result in the Scopus database

2-s2.0-85208636396