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The Mysterious World of Non-Canonical Caps – What We Know and Why We Need New Sequencing Techniques

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00600504" target="_blank" >RIV/61388963:_____/25:00600504 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/25:10506215

  • Result on the web

    <a href="https://doi.org/10.1002/cbic.202400604" target="_blank" >https://doi.org/10.1002/cbic.202400604</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/cbic.202400604" target="_blank" >10.1002/cbic.202400604</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The Mysterious World of Non-Canonical Caps – What We Know and Why We Need New Sequencing Techniques

  • Original language description

    It was long believed that viral and eukaryotic mRNA molecules are capped at their 5' end solely by the N7-methylguanosine cap, which regulates various aspects of the RNA life cycle, from its biogenesis to its decay. However, the recent discovery of a variety of non-canonical RNA caps derived from metabolites and cofactors such as NAD, FAD, CoA, UDP-glucose, UDP-N-acetylglucosamine, and dinucleoside polyphosphates has expanded the known repertoire of RNA modifications. These non-canonical caps are found across all domains of life and can impact multiple aspects of RNA metabolism, including stability, translation initiation, and cellular stress responses. The study of these modifications has been facilitated by sophisticated methodologies such as liquid chromatography-mass spectrometry, which have unveiled their presence in both prokaryotic and eukaryotic organisms. The identification of these novel RNA caps highlights the need for advanced sequencing techniques to characterize the specific RNA types bearing these modifications and understand their roles in cellular processes. Unravelling the biological role of non-canonical RNA caps will provide insights into their contributions to gene expression, cellular adaptation, and evolutionary diversity. This review emphasizes the importance of these technological advancements in uncovering the complete spectrum of RNA modifications and their implications for living systems.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA for therapy</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chembiochem

  • ISSN

    1439-4227

  • e-ISSN

    1439-7633

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    e202400604

  • UT code for WoS article

    001342113800001

  • EID of the result in the Scopus database

    2-s2.0-85207710929