Discovery of metabolites prevails amid in-source fragmentation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00618108" target="_blank" >RIV/61388963:_____/25:00618108 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1038/s42255-025-01239-4" target="_blank" >https://doi.org/10.1038/s42255-025-01239-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42255-025-01239-4" target="_blank" >10.1038/s42255-025-01239-4</a>

Result language
angličtina
Original language name
Discovery of metabolites prevails amid in-source fragmentation
Original language description
Recent claims suggest that most unannotated signals in untargeted LC–MS metabolomics arise from in-source fragmentation (ISF), implying that the “dark metabolome” may be largely a technical artifact. Here, we argue that this interpretation is overstated and not sufficiently supported by publicly available data. Our analyses indicate that ISFs are typically few and of low intensity relative to precursor ions, and that modern data-processing workflows are capable of identifying and accounting for these ion forms. Furthermore, analysis of a well-characterized human fecal reference sample shows that the majority of detected molecular features remain unannotated even after grouping related ion forms. Together, these findings support the continued existence of a substantial dark metabolome and highlight ongoing opportunities for metabolite discovery.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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