Study of metabolic pathways of racemic ketamine and its (S)-enantiomer in rat blood plasma using CE-ESI/MS with partial filling of dual chiral selector system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00619154" target="_blank" >RIV/61388963:_____/25:00619154 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/25:43928372
Result on the web
<a href="https://doi.org/10.1016/j.talanta.2025.128129" target="_blank" >https://doi.org/10.1016/j.talanta.2025.128129</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2025.128129" target="_blank" >10.1016/j.talanta.2025.128129</a>
Alternative languages
Result language
angličtina
Original language name
Study of metabolic pathways of racemic ketamine and its (S)-enantiomer in rat blood plasma using CE-ESI/MS with partial filling of dual chiral selector system
Original language description
Ketamine is a chiral drug used as anesthetic, analgesic and antidepressant. Its enantiomers and stereoisomers of its metabolites show different pharmacological and behavioral effects. To study the ketamine metabolic pathway and investigate these effects, highly sensitive and enantioselective methods are required. For that reason, in this study, a new CE method using a partial filling dual chiral selector system and ESI-MS detection has been developed and applied for separation and quantification of enantiomers of ketamine and its main metabolites, norketamine, hydroxynorketamine and dehydronorketamine, extracted by dichloromethane from the blood plasma of laboratory rats. The dual chiral selector system consisting of two zones of highly sulfated β-cyclodextrin (30 mg mL−1) and highly sulfated γ-cyclodextrin (10 mg mL−1) was introduced consecutively near the capillary outlet end. Both chiral selectors were dissolved in the background electrolyte composed of 10 mM ammonium hydroxide, 104 mM acetic acid, 10 % (v/v) ethanol, pH∗ 3.75. This system enabled enantioseparation of ketamine and its metabolites within a single CE run. High resolutions (3.99–17.61) of enantiomers of all above four analytes within a short time (11 min) were achieved in the fused silica capillary covalently coated with weakly negatively charged polyanionic copolymer (poly(acrylamide-co-sodium-2-acrylamido-2-methylpropanesulfonate), PAMAMPS). This coating minimized analyte sorption to the capillary and provided good repeatability of migration times. The limits of detection and quantification of the above analytes were in the range 108–238 nM and 361–792 nM, respectively. The method was linear within wide concentration range of 0.1–200 μM and the recovery was 91.3–105 %.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA22-22398S" target="_blank" >GA22-22398S: Microfluidic and electronic devices for on-line electrophoretic analysis of adipose tissue</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Volume of the periodical
293
Issue of the periodical within the volume
October
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
128129
UT code for WoS article
001471776100001
EID of the result in the Scopus database
2-s2.0-105002411678