New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00645539" target="_blank" >RIV/61388963:_____/25:00645539 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00645539
Result on the web
<a href="https://doi.org/10.1007/s00335-025-10115-1" target="_blank" >https://doi.org/10.1007/s00335-025-10115-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00335-025-10115-1" target="_blank" >10.1007/s00335-025-10115-1</a>
Alternative languages
Result language
angličtina
Original language name
New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system
Original language description
nThe Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including the angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) across multiple organs, to investigate the effects of SARS-CoV-2 infection beyond the respiratory system. This strain demonstrated susceptibility to SARS-CoV-2 infection in a dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, including significant weight loss, pronounced lung pathology and dysfunction, and increased mortality, compared to females. In contrast to intratracheal infection, intranasal virus administration facilitated viral spread to the brain, thereby underscoring the nasal route's role in the pathogenesis of neurological manifestations. Intranasal infection also led to increased innate immune system activation as compared to intratracheal virus administration, even though both routes activated the adaptive immune response. This model provides a valuable tool to study SARS-CoV-2 in individual tissues or use a multisystemic approach, and it also advances possibilities for preclinical evaluation of antiviral therapies and vaccine strategies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mammalian Genome
ISSN
0938-8990
e-ISSN
1432-1777
Volume of the periodical
36
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
14
Pages from-to
403-416
UT code for WoS article
001427763500001
EID of the result in the Scopus database
2-s2.0-85218692509