E1F34/RPG1P affects reassembly of microtubules in Saccharomyces cerevisiae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00100382" target="_blank" >RIV/61388971:_____/04:00100382 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
E1F34/RPG1P affects reassembly of microtubules in Saccharomyces cerevisiae
Original language description
Rpg1p/Tif32p/eIF3a is the large subunit of the eIF3 core complex involved in translation initiation in Saccharomyces cerevisiae. We prepared and analzyed the phenotyp of a new mutant rpg1-2. Similarly to the rpg1-1 mutant, the mutant rpg1-2 arrests in the G1 phase but in contrast, it is unable to recover after the treatment with the microtubule disrupting drug nocodazole at the restrictive temperature 37°C. This phenotype is similar to the phenotype of the tcp1 deletion mutant what indicates a potentialrole of Rpg1p in the refolding of tubulin after the nocodazole treatment.
Czech name
E1F34/RPG1P ovlivňuje nové uspořádání mikrotubulů u Saccharomyces cerevisiae
Czech description
Rpg1p/Tif32p/eIF3a je velkou podjednotkou eIF3 komplexu kvasinky Saccharomyces cerevisiae. V této práci jsme analyzovali fenotyp mutantu rpg1-2. Podobně jako mutant rpg1-1 mutant, mutant rpg1-2 zastavuje v G1 fázi, ale na rozdíl od tohoto mutantu není schopen obnovy mikrotubulárního systému po uvolnění z nocodazolu v restriktivní teplotě. Tehto fenotyp je podobný fenotypu mutantu tcp1beta což naznačuje možnou ulohu Rpg1 v refoldingu tubulinu.
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA204%2F02%2F1424" target="_blank" >GA204/02/1424: Role of cytoskeletal interactions of the translation initiation factor eIF3 in yeast morphogenesis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Annual Conference on Yeasts /32./
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
32
Publisher name
Neuveden
Place of publication
Smolenice
Event location
Smolenice
Event date
May 12, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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