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E1F34/RPG1P affects reassembly of microtubules in Saccharomyces cerevisiae

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00100382" target="_blank" >RIV/61388971:_____/04:00100382 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    E1F34/RPG1P affects reassembly of microtubules in Saccharomyces cerevisiae

  • Original language description

    Rpg1p/Tif32p/eIF3a is the large subunit of the eIF3 core complex involved in translation initiation in Saccharomyces cerevisiae. We prepared and analzyed the phenotyp of a new mutant rpg1-2. Similarly to the rpg1-1 mutant, the mutant rpg1-2 arrests in the G1 phase but in contrast, it is unable to recover after the treatment with the microtubule disrupting drug nocodazole at the restrictive temperature 37°C. This phenotype is similar to the phenotype of the tcp1 deletion mutant what indicates a potentialrole of Rpg1p in the refolding of tubulin after the nocodazole treatment.

  • Czech name

    E1F34/RPG1P ovlivňuje nové uspořádání mikrotubulů u Saccharomyces cerevisiae

  • Czech description

    Rpg1p/Tif32p/eIF3a je velkou podjednotkou eIF3 komplexu kvasinky Saccharomyces cerevisiae. V této práci jsme analyzovali fenotyp mutantu rpg1-2. Podobně jako mutant rpg1-1 mutant, mutant rpg1-2 zastavuje v G1 fázi, ale na rozdíl od tohoto mutantu není schopen obnovy mikrotubulárního systému po uvolnění z nocodazolu v restriktivní teplotě. Tehto fenotyp je podobný fenotypu mutantu tcp1beta což naznačuje možnou ulohu Rpg1 v refoldingu tubulinu.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA204%2F02%2F1424" target="_blank" >GA204/02/1424: Role of cytoskeletal interactions of the translation initiation factor eIF3 in yeast morphogenesis</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Annual Conference on Yeasts /32./

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    32

  • Publisher name

    Neuveden

  • Place of publication

    Smolenice

  • Event location

    Smolenice

  • Event date

    May 12, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article