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Rpg1p/elF3a of Saccharomyces cerevisiae accumulates in stress granules

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00107929" target="_blank" >RIV/61388971:_____/04:00107929 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Rpg1p/elF3a of Saccharomyces cerevisiae accumulates in stress granules

  • Original language description

    Rpg1p/Tif32p/eIF3a is the large subunit of the eIF3 core complex involved in translation initiation in Saccharomyces cerevisiae.We found that in unstressed cells Rpg1-GFP fusion is uniformly distributed throughout the cytoplasm. Upon osmotic upshift theprotein rapidly accumulated in fluorescing foci dispersed in the cytoplasm. Accumulation of Rpg1-GFP fusion was observed near the tip of small buds and at the mother-daughter neck. The formation of Rpg1-GFP granules was not altered by disruption of microtubules or F-actin, and by the absence of Sla2p.We suggest that Rpg1p/eIF3a mediates formation and dissolution of stress granules.

  • Czech name

    Rpg1p/elF3a je v Saccharomyces cerevisiae akumulován ve stresových granulích

  • Czech description

    Rpg1p/Tif32p/eIF3a je velká podjednotka eIF3 komplexu fungujícího v iniciaci translace v Saccharomyces cerevisiae.Zjistili jsme, že v nestresovaných buňkách obě, jak GFP tak i mRFP fůze Rpg1p/eIF3a byly rovnoměrně distribuovány v cytoplazmě. Po teplotnímšoku Rpg1p/eIF3a akumuloval ve fluoreskujících granulích ve vrcholu pupene a v krčku. Tvorba granulí nebyla závislá na přítomnosti mikrotubulů a aktinu, a nezávisí na Sla2. Předpokládáme, že Rpg1p/eIF3a zprostředkuje tvorbu a rozpuštění stresových granulí.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA204%2F02%2F1424" target="_blank" >GA204/02/1424: Role of cytoskeletal interactions of the translation initiation factor eIF3 in yeast morphogenesis</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Yeast Genetics and Molecular Biology Meeting /2004./

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    106

  • Publisher name

    Neuveden

  • Place of publication

    Seattle

  • Event location

    Seattle

  • Event date

    Jul 27, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article