Robust heat shock induces eIF2?-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F09%3A00326270" target="_blank" >RIV/61388971:_____/09:00326270 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Robust heat shock induces eIF2?-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae

Original language description

Formation of stres granules (SGs) has not yet been described in S. cerevisiae. We showed that robust heat shock induced accumulation of eIF3a/Tif32p/Rpg1p in cytoplasmic foci. They also contained eIF3b/Prt1p, eIF3c/Nip1p, eIF4G2, Pab1p, mRNA and small but not large ribosomes and eIF2a/Sui2p. Surprisingly, Dcp2p and Dhh1p P-body markers also co-localized with eIF3a, however, since formation of eIF3-accumulations required different scaffolding proteins than P-bodies, we suggest that they represent yeast specific SGs

Czech name

Robustní teplotní stres nezávisle na fosforylaci eIF2alfa indukuje v kvasince Saccharomyces cerevisiae tvorbu stresových granulí obsahujících eIF3 a 40S ribosomální podjednotku

Czech description

Tvorba stresových granulí v kvasince Saccharomyces cerevisiae dosud nebyla popsána. Ukázali jsme, že robustní teplotní stres indukuje akumulaci eIF3a/Tif32p/Rpg1p v cytoplazmatických ohniscích. Ty také obsahují eIF3b/Prt1p, eIF3c/Nip1p, eIF4G2, Pab1p, mRNA a malou, ale ne velkou ribosomální podjednotku a faktor eIF2a/Sui2. V těchto místech se překvapivě také nachází proteiny P-bodies Dcp2 a Dhh1. Jelikož však akumulace eIF3 vyžaduje jiné strukturální proteiny než P-bodies, považujeme vznikající akumulace eIF3a za stresové granule

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

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Volume of the periodical

122

Issue of the periodical within the volume

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Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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