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High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00107921" target="_blank" >RIV/61388971:_____/04:00107921 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer

  • Original language description

    Expression of a recombinant pyranose oxidase (P2O) from the basidiomycete Trametes ochracea in recombinant Escherichia coli strain was studied in batch and fed-batch cultivations. The P2O productivity has been improved using the fed-batch strategy and the optimized culture medium. The purified recombinant P2O was found to be similar to the P2O isolated from its original host.

  • Czech name

    Vysoká úroveň exprese houbové pyranosooxidasy při ?fed-batch? kultivacích Escherichia coli do vysoké density při použití laktosy jako induktoru

  • Czech description

    Byla studována exprese rekombinantní pyranosooxidasy (P2O) z basidiomycety Trametes versicolor v rekombinantní Escherichia coli. Produktivita P2O byla zlepšeny při použití kultivace "fed batch" a optimalizací kultivačního média. Purifikovaná rekombinantní P2O se neliší PSO izolované z původního hostitele.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Protein Expression and Purification

  • ISSN

    1046-5928

  • e-ISSN

  • Volume of the periodical

    36

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    61-69

  • UT code for WoS article

  • EID of the result in the Scopus database