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Expression of the pyranose 2-oxidase from Trametes pubescens in Escherichia coli and characterization of the recombinant enzyme

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F05%3A00023475" target="_blank" >RIV/61388971:_____/05:00023475 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Expression of the pyranose 2-oxidase from Trametes pubescens in Escherichia coli and characterization of the recombinant enzyme

  • Original language description

    c-DNA-encoding pyranose 2-oxidase (P2O) from Trametes pubescens was sequenced and cloned into Escherichia coli strain. The constitutive synhesis of P2O was studied in a batch culture in M9-based mineral medium. Purified recombinant enzyme has similar physico-chemical and kinetic parameters with other P2Os

  • Czech name

    Exprese pyranosa 2-oxidasy z Trametes pubescens v Escherichia Coli a charakterizace rekombinantního enzymu

  • Czech description

    c-DNA kódující pyranosa 2-oxidasu (P2O) z Trametes pubescens byla sekvenována a klonována v Escherichia coli. Konstitutivní syntéza P2O byla studována v jednorázových kulturách na minerálním M9 médiu. Purifikovaný rekombinantní enzym má podobné fyzikálně-chemické a kinetické parametry s ostatními pyranosaoxidasami

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Biotechnology

  • ISSN

    0168-1656

  • e-ISSN

  • Volume of the periodical

    120

  • Issue of the periodical within the volume

    -

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    9

  • Pages from-to

    387-395

  • UT code for WoS article

  • EID of the result in the Scopus database