Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00107943" target="_blank" >RIV/61388971:_____/04:00107943 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Enzymatic synthesis of complex glycosaminotrioses and study of their molecular recognition by hevein domains
Original language description
The trisaccharides GlcNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (1) and GalNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (2) were synthetised using beta-N-acetylhexosaminidase from Aspergillus oryzae. NMR and molecular modelling proved that both compounds are recognised by protein hevein, similarly to chitotriose (3). The association constants decrease in order 3, 1, 2.
Czech name
Enzymatická syntéza glykozaminotrióz a jejich molekulární identifikace pomocí herein domén
Czech description
Pomocí beta-N-acatylhexosaminidázy z Aspergillus oryzae byly syntetizovány trisacharidy GlcNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (1) a GalNAc-beta(1-4)-GlcNAc-beta(1-4)ManNAc (2). Jejich vazba na protein hevein byla spolu s chitobiosou (3) prokázána NMR amolekulárním modelováním. Asociační konstanty klesají v pořadí 3, 1, 2.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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