Purification and characterization of a nitrilase from Aspergillus niger K10

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00048555" target="_blank" >RIV/61388971:_____/06:00048555 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/06:10579 RIV/60461373:22330/06:00021417

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Purification and characterization of a nitrilase from Aspergillus niger K10

Original language description

Aspergillus niger K10 cultivated on 2-cyanopyridine produced high levels of an intracellular nitrilase, which was partially purified (18.6-fold) with a 24% yield. The N-terminal amino acid sequence of the enzyme was highly homologous with that of a putative nitrilase from Aspergillus fumigatus Af293. The enzyme was copurified with two proteins, the N-terminal amino acid sequences of which revealed high homology with those of hsp60 and an ubiquitin-conjugating enzyme. The nitrilase exhibited maximum activity (91.6 U mg-1) at 45 °C and pH 8.0. Its preferred substrates, in the descending order, were 4-cyanopyridine, benzonitrile, 1,4-dicyanobenzene, thiophen-2-acetonitrile, 3-chlorobenzonitrile, 3-cyanopyridine and 4-chlorobenzonitrile. Formation of amides as by-products was most intensive, in the descending order, for 2-cyanopyridine, 4-chlorobenzonitrile, 4-cyanopyridine, and 1,4-dicyanobenzene

Czech name

Purifikace a charakterizace nitrilasy z Aspergillus niger K10

Czech description

Aspergillus niger K10 kultivovaný na 2-kyanopyridinu produkoval velké množství intracelulární nitrilasy, která byla částečně purifikována (18,6krát) s 24% výtěžkem. N-terminální aminokyselinová sekvence enzymu byla vysoce homologní s pravděpodobnou nitrilasou z Aspergilus fumigatus Af293. Spolu s enzymem byly kopurifikovány další dva proteiny, jejichž N-terminální aminokyselinové sekvence byla vysoce homologní s hsp60 a ubiquitinconjugating enzymem A. fumigatus. Nitrilasa vykazovala maximum aktivity (91,6 U.mg-1) při 45 °C a pH 8,0. Jejími nejlepšími substráty byly v sestupném pořadí: 4-kyanopyridin, benzonitril, 1,4-dikyanobenzen, thiofen-2-acetonitril, 3-chlorbenzonitril, 3-kyanopyridin a 4-chlorbenzonitril. Tvorba amidů jako koproduktů byla nejintenzivnější (v sestupném pořadí) u 2-kyanopyridinu, 4-chlorbenzonitrilu, 4-kyanopyridinu a 1,4-dikyanobenzenu

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Volume of the periodical

73

Issue of the periodical within the volume

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Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

567-575

UT code for WoS article

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EID of the result in the Scopus database

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