Immobilization of fungal nitrilase and bacterial amidase ? two enzymes working in accord

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00051108" target="_blank" >RIV/61388971:_____/06:00051108 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immobilization of fungal nitrilase and bacterial amidase ? two enzymes working in accord

Original language description

A nitrilase from Aspergillus niger and an amidase from Rhodococcus erythropolis co-immobilized on a 1-mL Butyl Sepharose column were used for the hydrolysis of 4-cyanopyridine into isonicotinic acid. The former enzyme converted the nitrile into the acid: amide mixture (molar ratio ca. 3 : 1), while the latter enzyme hydrolyzed the by-product amide. Therefore, the ratio of amide in the total product decreased to about 5 %. Sodium sulfate was used as a component of the elution buffer, as the commonly used ammonium sulfate (0.8 M) acted as an amidase inhibitor. The hydrolysis of 4-cyanopyridine by a nitrilase from F. solani gave isonicotinic acid and isonicotinamide at a molar ratio of about 98 : 2. When using this enzyme and the amidase immobilized on two columns operated in tandem, the percentage of isonicotinamide in total product decreased to < 0.2 %kdi

Czech name

Imobilizace houbové nitrilasy a bakteriální amidasy ? součinnost dvou enzymů

Czech description

Nitrilasa z vláknité houby Aspergillus niger a amidasa z baktérie Rhodococcus erythropolis byly koimobilizovány na 1-mL koloně s Butyl Sepharosou a použity pro hydrolýzu 4-kyanopyridinu na kyselinu isonikotinovou. Nitrilasa transformovala nitril na směskyseliny a amidu (mol. poměr ca. 3 : 1), zatímco amidasa hydrolyzovala vedlejší produkt amid. Zastoupení amidu v celkovém produktu se tak snížilo na asi 5%. Síran sodný byl použit jako součást elučního pufru, protože obvykle používaný síran amonný (0,8 M) působil jako inhibitor amidasy. Hydrolýza 4-kyanopyridinu nitrilasou z F. solani poskytla kyselinu isonikotinovou a isonikotinamid v mol. poměru ca. 98 : 2. Když byly nitrilasa a amidasa imobilizovány na dvou kolonách spojených sériově, zastoupení isonikotinamidu v celkovém produktu se snížilo na < 0.2 %

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biocatalysis and Biotransformation

ISSN

1024-2422

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

414-418

UT code for WoS article

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EID of the result in the Scopus database

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