Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00050785" target="_blank" >RIV/61388971:_____/06:00050785 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/06:00006862 RIV/60076658:12640/06:00006780

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits

Original language description

Cyanobacteria contain several genes coding for small one-helix proteins called SCPs or HLIPs with significant sequence similarity to chlorophyll a/b-binding proteins. Using two-dimensional native-SDS electrophoresis of thylakoid membranes or isolated Photosystem II (PSII), we determined that after high-light treatment most of the ScpDHis protein in a cell is associated with PSII. The ScpDHis protein was present in both monomeric and dimeric PSII core complexes and also in the core subcomplex lacking CP43. However, the association with PSII was abolished in the mutant lacking the PSII subunit PsbH. In a PSII mutant lacking cytochrome b559, which does not accumulate PSII, ScpDHis is associated with CP47. The interaction of ScpDHis with PsbH and CP47 wasfurther confirmed by electron microscopy of PSII labeled with Ni-NTA Nanogold. Single particle image analysis identified the location of the labeled ScpDHis at the periphery of the PSII core complex in the vicinity of the PsbH and CP47

Czech name

Lokalizace malého chlorofyl-vážícího proteinu ScpD (HliB) v blízkosti dvou podjednotek PsbH a CP47 u fotosystemu II ze sinic

Czech description

Sinice obsahují několik genů, které kódují malé transmembránové proteiny. Tyto proteiny se nazývají SCP nebo HLIP a mají sekvenční podobnost s chlorofyl a/b-vážícími proteiny z vyšších rostlin. Pomocí dvourozměrné elektroforézy izolovaného jsme identifikovali většinu proteinu ScpD asociovaného s komplexem fotosystému II, a to jak s monomerní ale i dimerní formou. Interakce ScpD proteinu s fotosystemem II byla také studována pomocí elektronové mikroskopie se zlatem značenou afinitní značkou

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

281

Issue of the periodical within the volume

43

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

32705-32713

UT code for WoS article

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EID of the result in the Scopus database

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