Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00050802" target="_blank" >RIV/61388971:_____/06:00050802 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/06:00005072

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

Original language description

The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments and their reduction products were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. Based on their physical?chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen

Czech name

Identifikace redukčních produktů bilirubinu kmene Clostridium perfringens izolovaného z novorozenecké fekální flóry

Czech description

Kmen Clostridium perfringens izolovaný z novorozenecké fekální flóry byl použit v této studii. Pomocí spektrometrických metod byla analyzována schopnost metabolizovat rozmanité přírodní i syntetické žlučové pigmenty. Analýzou redukčních produktů vzniklých jejich konverzí jsme odhalili schopnost kmene BR1 metabolizovat dané pigmenty až na úroveň urobilinogenů, redukce na úroveň sterkobilinogenů však nepokračovala

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

<a href="/en/project/GA310%2F02%2F1436" target="_blank" >GA310/02/1436: Isolation and characterization of bilirubin reductase gene from bacteria of Clostridium genus and its potential therapeutic use</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

—

Volume of the periodical

833

Issue of the periodical within the volume

-

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

149-157

UT code for WoS article

—

EID of the result in the Scopus database

—