Cocaine- and amphetamine-regulated transcript (CART) peptide specific binding in pheochromocytoma cells PC12
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00084044" target="_blank" >RIV/61388971:_____/07:00084044 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/07:00084044 RIV/61388963:_____/07:00084044
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cocaine- and amphetamine-regulated transcript (CART) peptide specific binding in pheochromocytoma cells PC12
Original language description
We report specific binding of 125I-CART(61-102) to the rat adrenal pheochromocytoma PC12 cell line, both intact cells and cell membranes. Saturation binding to intact plated cells resulted in Kd of 0.48+-0.16 nM and Bmax of 2228+-529 binding sites/cell.In competitive binding experiments, CART(61-102), CART(55-102) and di-iodinated CART(61-102) had similar affinity to PC12 cell membranes, having Ki of 2.99+-0.65, 2.98+-0.33 and 1.27+-0.2 nM, respectively. 125I-CART(61-102) was also bound to intact PC12cells differentiated using nerve growth factor to the neuronal phenotype.
Czech name
Specifická vazba peptidu CART (cocaine- and amphetamine-regulated transcript) k pheochromocytomálním buňkám PC12
Czech description
Byla změřena specifická vazba 125I-CART(61-102) k buňkám PC12 z nádoru krysích nadledvin, intaktním buňkám I membránám. Saturační vazba k celým buňkám byla charakterizována Kd = 0.48+-0.16 nM a Bmax = 2228+-529 vazebných mist na buňku. V kompetitivních vazebných experimentech měl CART(61-102), CART(55-102) a di-jodovaný CART(61-102) podobnou afinitu. 125I-CART(61-102) se take vázal k buňkám PC12 diferencovaným v neurální fenotyp.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA303%2F05%2F0614" target="_blank" >GA303/05/0614: Central and peripheral pathways underlying feeding-related effects of cocaine and amphetamine regulated transcript (CART) peptides</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Pharmacology
ISSN
0014-2999
e-ISSN
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Volume of the periodical
559
Issue of the periodical within the volume
2/3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
109-114
UT code for WoS article
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EID of the result in the Scopus database
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