In Vivo Stabilization of Preinitiation Complexes by Formaldehyde Cross-Linking

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00088909" target="_blank" >RIV/61388971:_____/07:00088909 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In Vivo Stabilization of Preinitiation Complexes by Formaldehyde Cross-Linking
Original language description
Translation initiation starts with the formation of the 43S preinitiation complex (PIC) consisting of several soluble factors, including the ternary complex (TC; elF2-GTP-Met-tRNAi Met), which associate with the small ribosomal subunit. In the next step,mRNA is recruited to form the 48S PIC and the entire machinery starts scanning the 50 untranslated region of the mRNA until the AUG start codon is encountered. The most widely used method to separate 40S and 60S ribosomal subunits from soluble factors,monosomes and polysomes, is sucrose density centrifugation (SDC). Since PICs are intrinsically unstable complexes that cannot withstand the forces imposed by SDC, a stabilization agent must be employed to detect the association of factors with the 40S subunit after SDC. This was initially achieved by adding heparin (a highly sulfated glycosaminoglycan) directly to the breaking buffer of cells treated with cycloheximide (a translation elongation inhibitor
Czech name
Stabilizace pre-iniciačních komplexů in vivo pomocí formaldehydu
Czech description
Práce pojednáva o studiu inciace translace a specifické úloze translačních iniciačních faktorů eIF1, 2, 3 a 5

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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