eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00312545" target="_blank" >RIV/61388971:_____/08:00312545 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

eIF3a cooperates with sequences 5´of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA

Original language description

Yeast initiation factor eIF3 (eukaryotic initiation factor 3) has been implicated in multiple steps of translation initiation. Previously, we showed that the N-terminal domain (NTD) of eIF3a interacts with the small ribosomal protein RPS0A located near the mRNA exit channel, where eIF3 is proposed to reside. Here, we demonstrate that a partial deletion of the RPS0A-binding domain of eIF3a impairs translation initiation and reduces binding of eIF3 and associated eIFs to native preinitiation complexes invivo. Strikingly, it also severely blocks the induction of GCN4 translation that occurs via reinitiation. Detailed examination unveiled a novel reinitiation defect resulting from an inability of 40S ribosomes to resume scanning after terminating at the first upstream ORF (uORF1). Genetic analysis reveals a functional interaction between the eIF3a-NTD and sequences 5_ of uORF1 that is critically required to enhance reinitiation

Czech name

eIF3 hraje kritickou úlohu v procesu reiniciace, který řídí expresi kvasinkového genu GCN4, prostřednictvým interakce s 5? oblastí uORF1 nacházející se v netranslatované oblasti GCN4

Czech description

Práce pojednává o studiu iniciace translace a specifické úloze translačního iniciačního faktoru eIF3

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genes and Development

ISSN

0890-9369

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

-

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

000258878100012

EID of the result in the Scopus database

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