eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00547363" target="_blank" >RIV/61388971:_____/21:00547363 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/nar/article/49/15/8743/6342458" target="_blank" >https://academic.oup.com/nar/article/49/15/8743/6342458</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkab652" target="_blank" >10.1093/nar/gkab652</a>

Result language

angličtina

Original language name

eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast

Original language description

Translation reinitiation is a gene-specific translational control mechanism. It is characterized by the ability of short upstream ORFs to prevent full ribosomal recycling and allow the post-termination 40S subunit to resume traversing downstream for the next initiation event. It is well known that variable transcript-specific features of various uORFs and their prospective interactions with initiation factors lend them an unequivocal regulatory potential. Here, we investigated the proposed role of the major initiation scaffold protein eIF4G in reinitiation and its prospective interactions with uORF's cis-acting features in yeast. In analogy to the eIF3 complex, we found that eIF4G and eIF4A but not eIF4E (all constituting the eIF4F complex) are preferentially retained on ribosomes elongating and terminating on reinitiation-permissive uORFs. The loss of the eIF4G contact with eIF4A specifically increased this retention and, as a result, increased the efficiency of reinitiation on downstream initiation codons. Combining the eIF4A-binding mutation with that affecting the integrity of the eIF4G1-RNA2-binding domain eliminated this specificity and produced epistatic interaction with a mutation in one specific cis-acting feature. We conclude that similar to humans, eIF4G is retained on ribosomes elongating uORFs to control reinitiation also in yeast.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GX19-25821X" target="_blank" >GX19-25821X: Global and transcript-specific analysis of translational control in disease.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

1362-4962

Volume of the periodical

49

Issue of the periodical within the volume

15

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

8743-8756

UT code for WoS article

000697383500028

EID of the result in the Scopus database

2-s2.0-85116537343