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Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00483845" target="_blank" >RIV/61388971:_____/17:00483845 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/17:10361161

  • Result on the web

    <a href="http://dx.doi.org/10.1080/15476286.2017.1353863" target="_blank" >http://dx.doi.org/10.1080/15476286.2017.1353863</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1080/15476286.2017.1353863" target="_blank" >10.1080/15476286.2017.1353863</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?

  • Original language description

    Reinitiation after translation of short upstream ORFs (uORFs) represents one of the means of regulation of gene expression on the mRNA-specific level in response to changing environmental conditions. Over the years it has been shown-mainly in budding yeast-that its efficiency depends on cis-acting features occurring in sequences flanking reinitiation-permissive uORFs, the nature of their coding sequences, as well as protein factors acting in trans. We earlier demonstrated that the first two uORFs from the reinitiation-regulated yeast GCN4 mRNA leader carry specific structural elements in their 5 sequences that interact with the translation initiation factor eIF3 to prevent full ribosomal recycling post their translation. Actually, this interaction turned out to be instrumental in stabilizing the mRNA 40S post-termination complex, which is thus capable to eventually resume scanning and reinitiate on the next AUG start site downstream. Recently, we also provided important in vivo evidence strongly supporting the long-standing idea that to stimulate reinitiation, eIF3 has to remain bound to ribosomes elongating these uORFs until their stop codon has been reached. Here we examined the importance of eIF3 and sequences flanking uORF1 of the human functional homolog of yeast GCN4, ATF4, in stimulation of efficient reinitiation. We revealed that the molecular basis of the reinitiation mechanism is conserved between yeasts and humans.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10605 - Developmental biology

Result continuities

  • Project

    <a href="/en/project/GA15-10116S" target="_blank" >GA15-10116S: Crossing the boundaries: analysis of functional mRNA elements determining the mechanism of translational reinitiation in lower and higher eukaryotes.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    RNA biology

  • ISSN

    1547-6286

  • e-ISSN

  • Volume of the periodical

    14

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    1660-1667

  • UT code for WoS article

    000423291400005

  • EID of the result in the Scopus database

    2-s2.0-85038371436