Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00483845" target="_blank" >RIV/61388971:_____/17:00483845 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10361161
Result on the web
<a href="http://dx.doi.org/10.1080/15476286.2017.1353863" target="_blank" >http://dx.doi.org/10.1080/15476286.2017.1353863</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15476286.2017.1353863" target="_blank" >10.1080/15476286.2017.1353863</a>
Alternative languages
Result language
angličtina
Original language name
Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?
Original language description
Reinitiation after translation of short upstream ORFs (uORFs) represents one of the means of regulation of gene expression on the mRNA-specific level in response to changing environmental conditions. Over the years it has been shown-mainly in budding yeast-that its efficiency depends on cis-acting features occurring in sequences flanking reinitiation-permissive uORFs, the nature of their coding sequences, as well as protein factors acting in trans. We earlier demonstrated that the first two uORFs from the reinitiation-regulated yeast GCN4 mRNA leader carry specific structural elements in their 5 sequences that interact with the translation initiation factor eIF3 to prevent full ribosomal recycling post their translation. Actually, this interaction turned out to be instrumental in stabilizing the mRNA 40S post-termination complex, which is thus capable to eventually resume scanning and reinitiate on the next AUG start site downstream. Recently, we also provided important in vivo evidence strongly supporting the long-standing idea that to stimulate reinitiation, eIF3 has to remain bound to ribosomes elongating these uORFs until their stop codon has been reached. Here we examined the importance of eIF3 and sequences flanking uORF1 of the human functional homolog of yeast GCN4, ATF4, in stimulation of efficient reinitiation. We revealed that the molecular basis of the reinitiation mechanism is conserved between yeasts and humans.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10605 - Developmental biology
Result continuities
Project
<a href="/en/project/GA15-10116S" target="_blank" >GA15-10116S: Crossing the boundaries: analysis of functional mRNA elements determining the mechanism of translational reinitiation in lower and higher eukaryotes.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA biology
ISSN
1547-6286
e-ISSN
—
Volume of the periodical
14
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1660-1667
UT code for WoS article
000423291400005
EID of the result in the Scopus database
2-s2.0-85038371436