Fail-safe mechanism of GCN4 translational control-uORF2 promotes reinitiation by analogous mechanism to uORF1 and thus secures its key role in GCN4 expression

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00433445" target="_blank" >RIV/61388971:_____/14:00433445 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/nar/gku204" target="_blank" >http://dx.doi.org/10.1093/nar/gku204</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gku204" target="_blank" >10.1093/nar/gku204</a>

Result language
angličtina
Original language name
Fail-safe mechanism of GCN4 translational control-uORF2 promotes reinitiation by analogous mechanism to uORF1 and thus secures its key role in GCN4 expression
Original language description
One of the extensively studied mechanisms of genespecific translational regulation is reinitiation. It takes place on messenger RNAs (mRNAs) where main ORF is preceded by upstream ORF (uORF). Even though uORFs generally down-regulate main ORF expression,specific uORFs exist that allow high level of downstream ORF expression. The key is their ability to retain 40S subunits on mRNA upon termination of their translation to resume scanning for the next AUG. Here, we took advantage of the exemplary model system of reinitiation, the mRNA of yeast transcriptional activator GCN4 containing four short uORFs, and show that contrary to previous reports, not only the first but the first two of its uORFs allow efficient reinitiation. Strikingly, we demonstrate that they utilize a similar molecular mechanism relying on several cis-acting 5_ reinitiation-promoting elements, one of which they share, and the interaction with the a/TIF32 subunit of translation initiation factor eIF3. Since a similar me
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GAP305%2F11%2F0172" target="_blank" >GAP305/11/0172: Molecular basis of the gene-specific translational control mechanism of yeast GCN4 and its mammalian functional homologue ATF4.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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