Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsA-inactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00312706" target="_blank" >RIV/61388971:_____/08:00312706 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12640/08:00009350

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Role of phosphatidylglycerol in the function and assembly of Photosystem II reaction center, studied in a cdsA-inactivated PAL mutant strain of Synechocystis sp. PCC6803 that lacks phycobilisomes

Original language description

The role of phosphatidylglycerol (PG) in photosynthetic membranes of cyanobacteria was studied in the Synechocystis sp. PCC6803 PAL mutant with missing phycobilisomes and with inactivated cdsA gene encoding cytidine 5-diphosphate diacylglycerol synthase,a key enzyme in PG synthesis. In a medium supplemented with PG the PAL/dcdsA mutant cells grew photoautotrophically. Depletion of PG in the medium resulted (a) in an arrest of cell growth and division, (b) in a slowdown of electron transfer from the acceptor QA to QB in PSII and (c) in a modification of chlorophyll fluorescence curve. Two-dimensional PAGE showed that in the absence of PG (a) the PSII dimer was decomposed into monomers, and (b) the CP43 protein was detached from a major part of the PSIIcore complex

Czech name

Role phosphatidylglycerolu ve funkci a skládání reakčního centra fotosystému II studovaná v mutantu PAL sinice Synechocystis PCC6803 s chybějícími fykobilisomy a s inaktivovaným genem cdsA

Czech description

Role phosphatidylglycerolu (PG) ve fotosyntetických membránách sinic jsme studovali v mutantu sinice Synechocystis sp. PCC6803 s chybějícími fykobilisomys a s inaktivovaným genem cdsA kódujícím cytidine 5-diphosphate diacylglycerol synthasu, klíčový enzym biosyntézy PG. PAL/dcdsA mutant rostl fotoautototrofně v médiu s dodaným PG, ale odstranění PG vedlo k: (a) zastavení růstu a dělení buněků (b) k zpomalení elektronového transportu mezi akceptory QA a QB ve fotosystému II (PSII) a(c) modifikaci of chlorofylových fluorescenčních křivek. Dvourozměrná elektroforéza ukázala, že absence PG vede (a) k rozpadu dimerní formy PSII na monomery, a (b) k odtržení CP43 od zbylé části komplexu PSII

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/IAA400200801" target="_blank" >IAA400200801: FtsH proteases and their function in the cyanobacterium Synechocystis PCC 6803</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

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Volume of the periodical

1777

Issue of the periodical within the volume

9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

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UT code for WoS article

000259287200013

EID of the result in the Scopus database

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