The C-terminal extension of ferrochelatase is critical for enzyme activity and for functioning of the tetrapyrrole pathway in Synechocystis strain PCC 6803
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00315013" target="_blank" >RIV/61388971:_____/08:00315013 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12640/08:00009368
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The C-terminal extension of ferrochelatase is critical for enzyme activity and for functioning of the tetrapyrrole pathway in Synechocystis strain PCC 6803
Original language description
We analysed the DH324 strain of Synechocystis sp. PCC 6803, which contains a truncated ferrochelatase enzyme lacking this C-terminal domain. Truncated FeCH was localized to the membrane fraction suggesting that the C-terminal domain is not necessary formembrane association of the enzyme. Measurements of enzyme activity and complementation experiments revealed that the DH324 mutation dramatically reduced activity of the FeCH. Moreover, the H324 mutant accumulated a large amount of protoporphyrin and levels of chlorophyll precursors were also significantly increased. Analysis of the recombinant full-length and truncated FeCHs demonstrated that the C-terminal extension is critical for activity of the FeCH and it is strictly required for oligomerization of this enzyme
Czech name
C-terminální extenze enzymu ferochelatázy je nezbytná pro aktivitu enzymu a pro fungování biosyntetické dráhy tetrapyrolů u Synechocystis PCC6830
Czech description
Analyzovali jsme kmen sinice Synechocystis sp. PCC 6803, obsahující zkrácenou formu enzymu ferochelatázy bez C-koncové domény. Zkráceny enzym byl lokalizován v membránové frakci, což ukazuje, že doména není nezbytná pro asociaci enzymu s membránou. Z měření aktivit a z komplementace vyplývalo, že mutace H324 dramaticky redukuje aktivitu ferochelatázy. Mimoto, mutant DH324 akumuloval velká množství protoporfyrinu and prekurzorů chlorofylu. Analýza rekombinantní úplné a zkrácené ferochelatatázy prokázala,že C-koncová doména je nezbytná pro aktivitu enzymu and pro jeho oligomerizaci
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Bacteriology
ISSN
0021-9193
e-ISSN
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Volume of the periodical
190
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
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UT code for WoS article
000253825500027
EID of the result in the Scopus database
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