Glycosidases: a key to tailored carbohydrates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F09%3A00327136" target="_blank" >RIV/61388971:_____/09:00327136 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/09:10109413

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Glycosidases: a key to tailored carbohydrates

Original language description

In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidasecatalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced glucuronyl synthases, an inverting xylosynthase and the first mutant endo-b-N-acetylglucosaminidase. A thorough selectionof enzyme strains and aptly modified substrates have resulted in rare glycostructures, such as N-acetyl-b-galactosaminuronates, b1,4-linked mannosides and a1,4-linked galactosides. The efficient selection of mutant enzymes is facilitated by high-throughput screening assays involving the co-expression of coupled enzymes or chemical complementation

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Trends in Biotechnology

ISSN

0167-7799

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000265136100003

EID of the result in the Scopus database

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