Investigating the early stages of Photosystem II assembly in Synechocystis sp. PCC 6803: isolation of CP47 and CP43 complexe
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00362744" target="_blank" >RIV/61388971:_____/11:00362744 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M110.207944" target="_blank" >http://dx.doi.org/10.1074/jbc.M110.207944</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M110.207944" target="_blank" >10.1074/jbc.M110.207944</a>
Alternative languages
Result language
angličtina
Original language name
Investigating the early stages of Photosystem II assembly in Synechocystis sp. PCC 6803: isolation of CP47 and CP43 complexe
Original language description
Using the cyanobacterium Synechocystis sp. PCC 6803, we describe here the isolation of the CP47 and CP43 subunits, which, during biogenesis, attach to a reaction center assembly complex containing D1, D2, and cytochrome b559, with CP47 binding first. Absorption spectroscopy and pigment analyses revealed that both CP47-His and CP43-His bind chlorophyll a and âcarotene. A comparison of the low temperature absorption and fluorescence spectra in theQY region for CP47-His and CP43-His with those for CP47 andCP43 isolated by fragmentation of spinach PSII core complexes confirmed that the spectroscopic properties are similar but not identical. Immunoblotting and mass spectrometry revealed the co-purification of PsbH, PsbL, and PsbT with CP47-His and of PsbKand Psb30/Ycf12 with CP43-His. Overall, our data support the view that CP47 and CP43 form preassembled pigment-protein complexes in vivo before their incorporation into the PSII complex
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA400200801" target="_blank" >IAA400200801: FtsH proteases and their function in the cyanobacterium Synechocystis PCC 6803</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
286
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
14812-14819
UT code for WoS article
000289788300011
EID of the result in the Scopus database
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