Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00393101" target="_blank" >RIV/61388971:_____/13:00393101 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/jam.12057" target="_blank" >http://dx.doi.org/10.1111/jam.12057</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jam.12057" target="_blank" >10.1111/jam.12057</a>
Alternative languages
Result language
angličtina
Original language name
Long PCR-RFLP of 16S-ITS-23S rRNA genes: a high-resolution molecular tool for bacterial genotyping
Original language description
Aims To perform a systematic evaluation of the applicability, validity and reliability of the long PCR-RFLP of 16S-ITS-23S rRNA genes for bacterial genotyping using both sequences retrieved from public genome databases and the experimental data obtainedon bacterial cultures. Methods and Results 3301 Full-length sequences of 16S-ITS-23S rRNA genes were retrieved from 885 published bacterial genomes. Copy numbers of the whole set of 16S-ITS-23S rRNA genes per genome ranged from 1 (n = 161) to 14 (n = 4)with an average of 3.71. Their length varied greatly, from 4319 to 6568 bp with an average of 4952 bp. Computer-simulated RFLP analyses of the 16S-ITS-23S fragments flanked by the conserved primers 27F and 2241R suggested MspI, RsaI, HhaI and TaqI as themost appropriate enzymes for long PCR-RFLP analysis of the 16S-ITS-23S sequence
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F03.0110" target="_blank" >ED2.1.00/03.0110: Center of Algal Biotechnology Třeboň (Algatech)</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Applied Microbiology
ISSN
1364-5072
e-ISSN
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Volume of the periodical
114
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
433-447
UT code for WoS article
000313723800015
EID of the result in the Scopus database
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