Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00399053" target="_blank" >RIV/61388971:_____/13:00399053 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00572-012-0475-6" target="_blank" >http://dx.doi.org/10.1007/s00572-012-0475-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00572-012-0475-6" target="_blank" >10.1007/s00572-012-0475-6</a>

Result language

angličtina

Original language name

Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach

Original language description

A quantitative real-time PCR (qPCR) marker Ta0 with hydrolysis probe ("TaqMan"), targeted to the internal transcribed spacer region of the ribosomal DNA, has been developed for quantification of summer truffle (Tuber aestivum) mycelium. Gene copy concentrations determined by the qPCR were calibrated against pure culture mycelium of T. aestivum, enabling quantification of the mycelium in soil and in host roots from the fields. Significant concentrations of the fungus were observed not only in the finestroots with ectomycorrhizae but also in other root types, indicating that the fungus is an important component of the microbial film at the root surface. The concentration of T. aestivum in soil is relatively high compared to other ectomycorrhizal fungi.To evaluate the reliability of the measurement of the soil mycelium density using qPCR, the steady basal extracellular concentration of the stabilized T. aestivum DNA should be known and taken into account. Therefore, we addressed the sta

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GAP504%2F10%2F0382" target="_blank" >GAP504/10/0382: Tuber aestivum Vitt.: a study of distribution, biology and cultivation with the perspective of future practical exploitation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mycorrhiza

ISSN

0940-6360

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

341-348

UT code for WoS article

000320329000001

EID of the result in the Scopus database

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