The RNA Chaperone Hfq Is Required for Virulence of Bordetella pertussis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00399902" target="_blank" >RIV/61388971:_____/13:00399902 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1128/IAI.00345-13" target="_blank" >http://dx.doi.org/10.1128/IAI.00345-13</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/IAI.00345-13" target="_blank" >10.1128/IAI.00345-13</a>

Result language

angličtina

Original language name

The RNA Chaperone Hfq Is Required for Virulence of Bordetella pertussis

Original language description

Bordetella pertussis is a Gram-negative pathogen causing the human respiratory disease called pertussis or whooping cough. Here we examined the role of the RNA chaperone Hfq in B. pertussis virulence. Hfq mediates interactions between small regulatory RNAs and their mRNA targets and thus plays an important role in posttranscriptional regulation of many cellular processes in bacteria, including production of virulence factors. We characterized an hfq deletion mutant (Delta hfq) of B. pertussis 18323 andshow that the Delta hfq strain produces decreased amounts of the adenylate cyclase toxin that plays a central role in B. pertussis virulence. Production of pertussis toxin and filamentous hemagglutinin was affected to a lesser extent. In vitro, the ability of the Delta hfq strain to survive within macrophages was significantly reduced compared to that of the wild-type (wt) strain. The virulence of the Delta hfq strain in the mouse respiratory model of infection was attenuated, with its c

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Infection and Immunity

ISSN

0019-9567

e-ISSN

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Volume of the periodical

81

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

4081-4090

UT code for WoS article

000325719900013

EID of the result in the Scopus database

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