Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00426259" target="_blank" >RIV/61388971:_____/13:00426259 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ibiod.2012.05.017" target="_blank" >http://dx.doi.org/10.1016/j.ibiod.2012.05.017</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ibiod.2012.05.017" target="_blank" >10.1016/j.ibiod.2012.05.017</a>

Result language
angličtina
Original language name
Biodegradation of phenol using recombinant plasmid-carrying Rhodococcus erythropolis strains
Original language description
The aim of the study was to utilize current knowledge of the phenol catabolic genes in Rhodococcus erythropolis CCM2595 to construct improved phenol degraders and test their bioremediation potential using real industrial wastewater. The genes pheA2A1-pheR-catR-catABC, coding for enzymes of the phenol degradation pathway and transcriptional regulators in R. erythropolis CCM2595, were cloned in various combinations in the multicopy R. erythropolis plasmid vector pSRK21 and the resulting constructs were introduced into R. erythropolis cells. All plasmid-carrying strains showed increased phenol hydroxylase activity. The strains harboring constructs pSRKAphe-cat and pSRKBphe-cat (both carrying all enzyme-coding genes of the phenol catabolic gene cluster) exhibited the highest phenol hydroxylase activities and proved to be the most efficient phenol degraders in minimal medium with 0.3 g L-1 phenol. The recombinant strains were up to 50% more efficient than the wild-type strain in the bioreme
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/2B08062" target="_blank" >2B08062: Genetic and physiological manipulations in bacterial degraders of aromatic pollutants and their application</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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