Functional and Biochemical Characterization of Human Eukaryotic Translation Initiation Factor 3 in Living Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00433442" target="_blank" >RIV/61388971:_____/14:00433442 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/14:00433442
Result on the web
<a href="http://dx.doi.org/10.1128/MCB.00663-14" target="_blank" >http://dx.doi.org/10.1128/MCB.00663-14</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/MCB.00663-14" target="_blank" >10.1128/MCB.00663-14</a>
Alternative languages
Result language
angličtina
Original language name
Functional and Biochemical Characterization of Human Eukaryotic Translation Initiation Factor 3 in Living Cells
Original language description
The main role of the translation initiation factor 3 (eIF3) is to orchestrate formation of 43S-48S preinitiation complexes (PICs). Until now, most of our knowledge on eIF3 functional contribution to regulation of gene expression comes from yeast studies.Hence, here we developed several novel in vivo assays to monitor the integrity of the 13-subunit human eIF3 complex, defects in assembly of 43S PICs, efficiency of mRNA recruitment, and postassembly events such as AUG recognition. We knocked down expression of the PCI domain-containing eIF3c and eIF3a subunits and of eIF3j in human HeLa and HEK293 cells and analyzed the functional consequences. Whereas eIF3j downregulation had barely any effect and eIF3a knockdown disintegrated the entire eIF3 complex,eIF3c knockdown produced a separate assembly of the a, b, g, and i subunits (closely resembling the yeast evolutionary conserved eIF3 core), which preserved relatively high 40S binding affinity and an ability to promote mRNA recruitment
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP305%2F10%2F0335" target="_blank" >GAP305/10/0335: Deciphering molecular details of the stringent decoding of the AUG start codon during eukaryotic translation initiation.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Biology
ISSN
0270-7306
e-ISSN
—
Volume of the periodical
34
Issue of the periodical within the volume
16
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
3041-3052
UT code for WoS article
000339381000007
EID of the result in the Scopus database
—