The Arabidopsis mitogen-activated protein kinase 6 is associated with ?-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00447446" target="_blank" >RIV/61388971:_____/15:00447446 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/nph.13501" target="_blank" >http://dx.doi.org/10.1111/nph.13501</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.13501" target="_blank" >10.1111/nph.13501</a>

Result language
angličtina
Original language name
The Arabidopsis mitogen-activated protein kinase 6 is associated with ?-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress
Original language description
Stress-activated plant mitogen-activated protein (MAP) kinase pathways play roles in growth adaptation to the environment by modulating cell division through cytoskeletal regulation, but the mechanisms are poorly understood. We performed protein interaction and phosphorylation experiments with cytoskeletal proteins, mass spectrometric identification of MPK6 complexes and immunofluorescence analyses of the microtubular cytoskeleton of mitotic cells using wild-type, mpk6-2 mutant and plants overexpressingthe MAP kinase-inactivating phosphatase, AP2C3. We showed that MPK6 interacted with -tubulin and co-sedimented with plant microtubules polymerized invitro. It was the active form of MAP kinase that was enriched with microtubules and followed similar dynamics to -tubulin, moving from poles to midzone during the anaphase-to-telophase transition. We found a novel substrate for MPK6, the microtubule plus end protein, EB1c. The mpk6-2 mutant was sensitive to 3-nitro-l-tyrosine (NO2-Tyr) trea
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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